IMMU 023
Flow Cytometry: Principles and Methods

Flow cytometry has been a fundamental technology utilized for cell counting, cell sorting, biomarker detection, and protein engineering. This lecture and laboratory workshop will cover various applications of flow cytometry in research. Selected lecture topics will provide a broad background about the functions of a flow cytometer, choice of fluorochromes, data analysis and presentation, technical protocols for flow cytometric procedures and troubleshooting during data acquisition and analysis. The lab sessions will include demonstrations of a few different flow cytometer instruments, along with hands-on training on FlowJo software.