Course Description

This course will introduce the basics of next generation bulk and single cell RNA sequencing data analysis. We will introduce the file formats, bioinformatics tools and standards, and data analysis workflows. The lectures will introduce common software libraries and algorithms used in the bioinformatics community. During the hands-on training sessions, we will perform alignment and analysis of raw datasets from scratch using standard software libraries and pipelines.



  • Participants will start with a general introduction to working in a linux environment and running command-line tools. We will learn the common file formats: fasta, fastq, SAM, BAM
  • Next, we will walk through:
    • Alignment and data visualization
    • Library normalization and downstream analysis in R
  • Finally, we will touch on accessing public data, retrieval methods, and best practices for making your analysis readable and reproducible.


Basic computing skills; a background in Linux and bash scripting is not required, though preferred. Experience with R is expected.

Target Audience (if any)

Although no grades are given for courses, each participant will receive Continuing Education Units (CEUs) based on the number of contact hours. One CEU is equal to ten contact hours. Upon completion of this course each participant will receive a certificate, showing completion of the workshop and 2.8 CEUs.

Refund Policy
100% tuition refund for registrations cancelled 14 or more calendar days prior to the start of the workshop.

50% tuition refund for registrations cancelled between 4 to 13 calendar days prior to the start of the workshop.

No refund will be issued for registrations cancelled 3 calendar days or less prior to the start of the workshop.

All cancellations must be received in writing via email to Ms. Carline Coote at registrar@faes.org.

Cancellations received after 4:00 pm (ET) on business days or received on non-business days are time marked for the following business day.

All refund payments will be processed by the start of the initial workshop.

Thank you for your interest in this course. Unfortunately, the course you have selected is currently not open for enrollment. Please complete a Course Inquiry so that we may promptly notify you when enrollment opens.
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